Induction of antibody response in the oral cavity of dogs followingintraocular (eye drop) immunization with Porphyromonas gingivalis celllysate incorporated in pH-sensitive fusogenic polymer-modified liposomes

[摘要] Induction of mucosal immune responses against Porphyromonas gingivaliswithin the oral cavity of dogs was studied by immunizing with pH-sensitive fusogenicpolymer (MGluPG)-modified liposome-associated cell lysate. Dogs immunized with P.gingivalis cell lysate-containing MGluPG-modified liposomes by intraocular (eyedrop) route displayed significant levels of P. gingivalis celllysate-specific serum IgG and IgA as well as mucosal IgA antibodies in saliva secretion.Serum and salivary antibodies generated by intraocularly immunized with MGluPG-modifiedliposome-associated P. gingivalis cell lysate revealed a significantaggregation activity against P. gingivalis, whereas serum and saliva fromdogs receiving MGluPG-modified liposomes unentrapping P. gingivalis celllysate did not show the aggregation activity against P. gingivalis.Furthermore, P. gingivalis-specific antibodies in saliva of immunizeddogs inhibited the adherence of P. gingivalis to cultured HeLa cells.More importantly, salivary antibodies induced by intraocular immunization with P.gingivalis cell lysate-containing MGluPG-modified liposomes significantlyinhibited the coaggregation of P. gingivalis with Actinomycesnaeslundii and the cell damage activity of P. gingivalisagainst FaDu cells, an oral epithelial cell. These results suggest that intraocularlyadministered P. gingivalis cell lysate-containing MGluPG-modifiedliposomes should be an effective mucosal vaccine against P. gingivalisinfection in dogs and may be an important tool for the prevention of periodontitis.

[学科分类] 兽医学

[关键词] intraocular immunization;liposome;mucosal immunity;periodontitis;Porphyromonas gingivalis