The occurrence of CTX-M-25-producing Enterobacteriaceae in day-old broilerchicks in Japan

[摘要] Day-old chicks from 3 hatcheries were placed on bedding paper and brought to a commercialbroiler farm between January and July 2016. Sixty-six samples of the paper, which werestained with meconium droppings of the chicks, were collected and examined for isolationof cephalosporin-resistant Enterobacteriaceae. Cefotaxime (CTX)-resistantKlebsiella pneumoniae (1 isolate) and Enterobactercloacae (4 isolates) were isolated from 5 (7.58%) of the 66 samples.Conjugation experiments revealed that the blaCTX-M-25 geneconferring CTX resistance was transferred from the K. pneumoniae isolateand 2 of the 4 E. cloacae isolates to Escherichia coliDH5α via IncA/C plasmids carrying the gene. Our results suggested that theblaCTX-M-25 gene originating from chicks may be spread amongcommercial broiler farms.

[学科分类] 兽医学

[关键词] antimicrobial resistance;chick;Enterobacteriaceae;ESBL