Evaluation Effect of Probiotic Cream in Carrageenan-Induced Inflammation Model in Male Rat Hind Paw

[摘要] Background:Inflammation is the first biological response produced by immune system against any infections or foreign allergen in order to protect the body. The main anti-inflammatory drugs that are used today are Non-steroidal anti-inflammatory drugs (NSAID), opioids, and corticosteroids. Despite the broad range of uses of these drugs, many side effects have been associated with their use such as digestive problems like ulcers and peptic bleeding. It has been suggested that products containing probiotics can be used as an effective anti-inflammatory drug, due to effects of Probiotics on immune responses such as reducing the inflammatory cytokines. In this study, we aim to investigate the anti-inflammatory effects of probiotics and to compare the effect with commonly used drugs such as Piroxicam and Calandula in an animal model.Method:In this experimental study, after preparation of topical probiotic cream, used Wistar male rats weighing 180-200 g were used and randomly divided into 6 groups; negative control, positive control (piroxicam & calandula) and 3 treatment groups (2%, 4% and 8% w/w of LPE). Three groups, each received different doses (2%, 4% and 8% w/w) of LPE cream by topical use was 30 minutes before injecting carrageenan into plantar side of hind paw of the rats. Piroxicam gel and Calandula ointment were studied as positive control for anti-inflammatory activity, respectively. Finally, to observe the anti-inflammatory effect, the rat paw edema was measured every 15 min in the first hour and then hourly for 5 hours. In the end, the volume of intact rat ҆s paw was measured in all groups by using plethysmometer. In the study, the IL-6 levels was measured by the ELIZA method.Result:All groups under the administration of the probiotic extract of Lactobacillus Casei compared to the negative control group in the rat paw edema show significant difference (P

[学科分类] 分析化学

[关键词] probiotic;lactobacillus casei;topical cream;carrageenan;anti-inflammatory;rat