Effects of Unilateral Iatrogenic Vas Deferens Trauma on Fertility: An Experimental In Vitro Fertilization Mice Model Study

[摘要] Objective:To determine bilateral effects of unilateral iatrogenic vas deferens trauma (UIT) on epididymal sperm characteristics andin vitrofertilizing capacity in an experimental mouse model. Methods:Experiments were performed on three equal groups each comprising six adult male mice. Following anaesthesia, UIT was induced by clamping left vas deferens with a mosquito clamp in fully locked fashion for 2 minutes in UIT group. Control-sham mice only had exposure of the left vas deferens through a groin incision. Control animals only received ceftriaxone (100 mg/kg) intraperitoneally at the day of experimental UIT induction. Ipsilateral and contralateral epididymal sperm characteristics andin vitrofertilizing capacity were evaluated after 35 days. Results:UIT significantly decreased sperm concentration, motility and viability as well as fertilization, two-cell embryos, blastocysts and hatched blastocysts rates. Moreover, incidence of DNA damage and abnormality in spermatozoa was significantly higher in UIT group. Conclusion:The findings suggest that a non-recognized iatrogenic vas deferens trauma may have detrimental effects on spermatozoa leading to infertility.

[学科分类] 基础医学

[关键词] In vitro;Fertilization;Mouse;Sperm;Trauma;Vas deferens