Mannose receptor and macrophage galactose‐type lectin are involved in Bordetella pertussis mast cell interaction

[摘要] MastcellsarecrucialinthedevelopmentofimmunityagainstBordetellapertussis,andthefunctionofTLRsinthisprocesshasbeeninvestigated.Here,theinteractionbetweenmastcellsandB.pertussiswithanemphasisontheroleofCLRsisexamined.Inthisstudy,twoCLRs,MGLandMR,weredetectedforthefirsttimeonthesurfaceofmastcells.TheinvolvementofMRandMGLinthestimulationofmastcellsbyheat‐inactivatedBPwasinvestigatedbytheuseofblockingantibodiesandspecificcarbohydrateligands.ThecellwallLOSofBPwasalsoisolatedtoexploreitsroleinthisinteraction.Mastcellsstimulatedwithheat‐inactivatedBPorBPLOSinducedTNF‐α,IL‐6,andIFN‐γsecretion,whichwassuppressedbyblockingMRorMGL.InhibitionofCLRssignalingduringBPstimulationaffectedtheabilityofmastcellstopromotecytokinesecretioninTcellsbuthadnoeffectonthecell‐surfaceexpressionofICAM1.BlockingMRorMGLsuppressedBP‐inducedNF‐κBexpressionbutnotERKphosphorylation.SykwasinvolvedintheCLR‐mediatedactivationofmastcellsbyBP.BacterialrecognitionbyimmunecellshasbeenpredominantlyattributedtoTLRs;inthisstudy,thenovelroleofCLRsintheBP–mastcellinteractionishighlighted...

[学科分类] 生理学

[关键词] C‐type lectin;cytokine;ICAM1;ERK;NF‐κB