Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics
[摘要] Inflammationisanindispensablecomponentoftheimmuneresponse,andleukocytesprovidethefirstlineofdefenseagainstinfection.Althoughthemajorstereotypicleukocytebehaviorsinresponsetoinfectionarewellknown,thecomplexitiesandidiosyncrasiesofthesephenotypesinconditionsofdiseasearestillemerging.Noveltoolsareindispensableforgaininginsightsintoleukocytebehavior,andinthepastdecade,microfluidictechnologieshaveemergedasanexcitingdevelopmentinthefield.Microfluidicdevicesarereadilycustomizable,providetightcontrolofexperimentalconditions,enablehighprecisionofexvivomeasurementsofindividualaswellasintegratedleukocytefunctions,andhavefacilitatedthediscoveryofnovelleukocytephenotypes.Here,wereviewsomeofthemostinterestinginsightsresultingfromtheapplicationofmicrofluidicapproachestothestudyoftheinflammatoryresponse.Theaimistoencourageleukocytebiologiststointegratethesenewtoolsintoincreasinglymoresophisticatedexperimentaldesignsforprobingcomplexleukocytefunctions...
[发布日期] [发布机构]
[效力级别] [学科分类] 生理学
[关键词] nanotechnology;inflammation;neutrophils;dendritic cells;monocytes [时效性]
